Phosphinothricin acetyltransferase

WebPhosphinothricin N-acetyltransferase (PAT) detoxifies the nonselective herbicide phosphinothricin (PPT, the ammonium salt of which is known as glufosinate), which is the active ingredient in ... WebApr 1, 2024 · Only few glufosinate-tolerant genes, such as phosphinothricin acetyltransferase (PAT) and bialaphos resistance (bar) identified from Streptomyces, are currently available for developing genetically modified rice in agricultural application.Following the rapid development of genome editing technology, generation of …

Resistance to glufosinate is proportional to …

WebFilters carrying the bombarded tissues were selected on basal medium with increasing concentrations (maximum of 200 mg I-1) of phosphinothricin. Surviving calli were assayed for the presence of phosphinothricin acetyltransferase activity. All positive calli were shown to have the bar gene integrated into their genome. Nine independent stable ... WebGmGSTU23 was then linearly cloned into the pTF101.1 binary vector with a modified CaMV 35S promoter, which contained a phosphinothricin acetyltransferase (bar) resistance gene (phosphinothricin N-acetyltransferase, PAT) as selection marker for the transgenic line. crystal river utilities https://b2galliance.com

Herbicide Combinations Comprising Glufosinate and Fluthiacet …

WebBovine phosphinothricin acetyltransferase (PAT) ELISA Kit. Detection Target: phosphinothricin acetyltransferase (PAT) Reactivity: Bovine. Quantity: 48-Strip-Wells. … WebDonn G, Dirks R, Eckes P, Uijtewaal B (1990a) Transfer and expression of modified phosphinothricin-acetyltransferase gene from Streptomyces viridichromogenes in tomato, melon, carrot, and strawberry. Abstr VII Int Congr Plant Tissue Cell Cult Amsterdam: 176. WebAbstract. Insertion of the gene encoding phosphinothricin acetyltransferase (PAT) has resulted in cotton plants resistant to the herbicide glufosinate. However, the lower … dying light sheep or wolf

Glufosinate - an overview ScienceDirect Topics

Category:Utility of the pat gene as a selectable marker gene in production of …

Tags:Phosphinothricin acetyltransferase

Phosphinothricin acetyltransferase

Stable transformation of barley callus using biolistic

WebDec 1, 1993 · Phosphinothricin acetyltransferase activity was detected in extracts of the T0 plants. These plants were resistant to local application of the herbicide Ignite/Basta, and the resistance was inherited in T1 plants as a single dominant locus. WebNov 21, 2016 · Acetylation of small molecules is widespread in nature, and in some cases, cells use this process to detoxify harmful chemicals. Streptomyces species utilize a Gcn5 …

Phosphinothricin acetyltransferase

Did you know?

WebPhosphinothricin Resistance against PTT can be achieved by elevated levels of GS, which protect bacteria from the action of PTT: the increased activity of GSII in S. viridochromogenes may be part of the self-protection mechanism against PTT. From: Encyclopedia of Microbiology (Third Edition), 2009 Tissues View all Topics Add to Mendeley

WebSep 10, 2016 · To examine the possibility of using the phosphinothricin N-acetyltransferase (pat) gene as a selectable marker gene, we introduced the pat genes into D. salina with particle bombardment system under the condition of helium pressure of 900 psi from a distance of 3 cm. PCR analysis confirmed that the gene was stably inserted into the cells … WebJun 15, 1992 · A 4-kb BamHI fragment of Streptomyces viridochromogenes Tü494 carrying phosphinothricin-tripeptide (PTT) biosynthetic genes has been identified by complementation of a nonproducing mutant which is defective in the tripeptide formation step. Nucleotide sequence analysis revealed one incomplete and three complete genes …

WebPhosphinothricin acetyltransferase (PAT) assays confirmed the expression of the bar gene in plants obtained from PPT-resistant calli. The only exceptions were two plants obtained from the same callus that had multiple copies of the bar gene integrated into their genomes. The transgenic status of the plants was varified by Southern blot analysis. Web2.2 Preparation of the Phosphinothricin Acetyltransferase Electrochemical Immunosensor Prior to modification, the GCE was polished with 1 μm, 0.3 µm, and 0.05 µm aluminum powder sequentially on polishing paper, with sonication in double-distilled water between each polishing step.

WebThe phosphinothricin acetyltransferase (PAT) proteins, which are encoded by the bar coding sequence from Streptomyces hygroscopicus or the pat coding sequence from …

WebApr 1, 2005 · DP23211 maize expresses an insect-specific dsRNA, an insect-active non-Bt protein, the phosphinothricin acetyltransferase (PAT) protein for tolerance to glufosinate herbicide, and the... dying light sign inWebphosphinothricin-N-acetyltransferase Test. Read more. Round up Ready cp4 Epsps protein (HerbicideResistant) ultra sensitive Test. Read more ... crystal river united methodist church floridaWebOct 1, 1996 · The bar and pat genes, isolated from different Streptomyces species, both encode a phosphinothricin acetyltransferase (PAT) and are widely applied in plant … crystal river toyota dealershipWebApr 11, 2024 · phosphinothricin acetyltransferase protein responsible for tolerance to glufosinate-ammonium herbicides. DP23211 corn also contains the gene that encodes for … crystal river tubing in floridaWebApr 11, 2024 · The Animal and Plant Health Inspection Service (APHIS) issued a final rule, published in the Federal Register on May 18, 2024 (85 FR 29790-29838, Docket No. APHIS-2024-0034) \1\, revising 7 CFR part 340; however, the final rule is being implemented in phases. The new Regulatory Status Review (RSR) process, which replaces the petition for ... dying light session no longer availableWebphosphinothricin-N-acetyltransferase Test. SKU: GENE-PHOS-NAT Category: Foody Safety Assays Tag: plantExtract. Related products. urinalysis test … dying light shield worth itWebMay 26, 2024 · Phosphinothricin acetyltransferase (PAT), the target protein (Kim et al. 2010) investigated in this study, is a typical herbicide-resistant protein coded by a bar gene isolated from Streptomyces hygroscopicus … dying light site rutracker.org