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Pacap receptor antibody western blot

WebAbstract. Rat pheochromocytoma PC12 cells have been widely used to investigate the neurotrophic activities of pituitary adenylate cyclase-activating polypeptide (PACAP). In particular, PACAP has been shown to promote differentiation and to inhibit apoptosis of PC12 cells. In order to identify the mechanisms mediating these effects, we sought ... WebWestern blot analyses were performed using the following primary rabbit polyclonal antibodies: PAC1 receptor (1:500, cat no# sc-30018, Santa Cruz Biotechnology Inc., Heidelberg, Germany), VPAC1 receptor (1:800, cat no# sc-30019, Santa Cruz Biotechnology Inc.), VPAC2 receptor (1:400, cat no# sc-30020, Santa Cruz Biotechnology Inc.), PACAP ...

PACAP Receptor 1/PAC1 Blocking Peptide BLP-VR003 - Alomone …

WebNov 11, 2002 · Pituitary adenylate cyclase activating polypeptide (PACAP) is a member of the vasoactive intestinal peptide ... The ability of PACAP-like peptides to phosphorylate MAPK was investigated using Western blot techniques . NCI-H1299 or NIH/3T3 cells were cultured in 15 cm dishes. ... Anti-VEGF monoclonal antibodies (mAbs) and the KDR/flk-1 … WebAnti-PACAP Receptor 1 (PAC1) Antibody (#AVR-003) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PAC1 receptor … my sweetheart where are you song https://b2galliance.com

PACAP receptor 1 Polyclonal Antibody (BS-0198R)

Webwww.antibodies-online.cn +1 877 302 8632 [email protected] Datasheet for ABIN6243291 ... 应用范围: Western Blotting (WB) ... Shows weak transcriptional activatory activity. Transcriptional regulator of the type 1 receptor for pituitary adenylate cyclase-activating polypeptide. WebThis is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of … WebV2 Receptor; VIP/PACAP Receptors. PAC1; Phospho-IHC 7TM Antibodies. Cannabinoid Receptors ... pT354-D1 Phosphorylation Assay Kit allows determination of D1 Phosphorylation in 96-well plates without the need for Western blot analysis. 300.00 € 600.00 € * Details ... ®7TM Antibodies is a registered trade mark in the US, EU and many … my sweetheart where are you i need someone

PACAP (6-38) is a PACAP receptor antagonist for breast cancer …

Category:Anti-PACAP receptor/ADCYAP1R1 antibody (ab183103)

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Pacap receptor antibody western blot

Frontiers Current State of Understanding of the Role of PACAP in …

WebThe PAC1 antibody is directed against the distal end of the carboxyl-terminal tail of human PACAP Receptor 1. It can be used to detect total PAC1 receptors in Western blots independent of phosphorylation. The PAC1 antibody can also be used to isolate and enrich PAC1 receptors from cell and tissue lysates. It also detects PAC1 in cultured cells ... WebFig. 1. Western blot analysis of M6PR on different cell lysates using anti-M6PR antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2; Lane 2: SW480 Fig. 2. ICC staining M6PR in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 /PBS. Fig. 3.

Pacap receptor antibody western blot

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WebBy RT-PCR, PACAP receptor mRNA was present in MCF-7 and T47D cell lines. Polyclonal antibodies to a PACAP receptor fragment (A-8-C) were elicited. The antibodies were … WebCompare Anti-PACAP Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. ... Western Blot (WB) Reactivity: Human (Hu) Conjugate/Tag: Unconjugated ... PACAP receptor/ADCYAP1R1 Antibody, Rabbit PAb, Antigen Affinity Purified. Applications: ICC, IF;

WebThe neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP) is a slow transmitter and a neuroendocrine hormone in the central and peripheral nervous system. It binds to its cognate G-protein-coupled receptor PAC1, elevating intracellular cAMP and calcium. PACAP protects neurons from excitotoxicity and hypoxic damage. WebThe blocking peptide binds and ‘blocks’ Anti-PACAP Receptor 1/PAC1 primary antibody, this makes it a good negative reagent control to help confirm antibody specificity in western blot and immunohistochemistry applications. This control is also often called a pre-adsorption control. Applications: wb, ihc Application key:

WebAnti-PACAP Receptor 1 (PAC1) Antibody (#AVR-003) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PAC1 receptor … WebĐăng nhập bằng facebook. Đăng nhập bằng google. Nhớ mật khẩu. Đăng nhập . Quên mật khẩu

WebBy RT-PCR, PACAP receptor mRNA was present in MCF-7 and T47D cell lines. Polyclonal antibodies to a PACAP receptor fragment (A-8-C) were elicited. The antibodies were affinity purified, recognized a 60-kDa protein by western blot, and stained malignant cells in breast cancer biopsy specimens by immunohistochemistry.

WebDetection of Human IFN‑ alpha / beta R1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human IFN‑ alpha / beta R1 Monoclonal Antibody (Catalog # MAB2452) followed by HRP-conjugated Anti-Rabbit ... my sweetheart\u0027sWebInvitrogen Anti-Cannabinoid Receptor 1 Polyclonal, Catalog # PA5-142759. Tested in Western Blot (WB) applications. This antibody reacts with Human, Mouse samples. Supplied as 100 µg purified antibody (0.5 mg/mL). the shops at palm desert hoursWebInvitrogen Anti-VPAC2 Polyclonal, Catalog # PA5-27162. Tested in Western Blot (WB) applications. This antibody reacts with Human samples. Supplied as 100 µL purified … the shops at overlookWebSee all PACAP receptor/ADCYAP1R1 primary antibodies Description Rabbit polyclonal to PACAP receptor/ADCYAP1R1 Host species Rabbit Tested applications Suitable for: IHC-P, WB more details Species reactivity Reacts with: Mouse, Human Immunogen Synthetic peptide within Human PACAP receptor/ADCYAP1R1 aa 1-100. the shops at palm desert mallWebPA3-213 detects PACAP type 1A receptor in rat and mouse samples. PA3-213 has successfully been used in Western blot and … the shops at palmer ranchmy sweetened condensed milk is brownWeb2.5. PACAP Receptor Inhibition Experiments. PC12 cells were adjusted to to cells/well on a 96-well plate and cultured for 24 hours and the PAC1-R inhibitor (PA-8: Professor Ichiro Takasaki, Faculty of Engineering, University of Toyama) [].VPAC1-R and VPAC2-R inhibitors (VIP6-28, V4508, Sigma-Aldrich) were added to the cell culture and incubated in a CO 2 … my sweetheart\u0027s the man in the moon